Reappraising heme oxygenase-1 as a ferroptosis modulator in atherosclerosis: a mechanism-focused review

Front Immunol. 2026 Feb 4:17:1737751. doi: 10.3389/fimmu.2026.1737751. eCollection 2026.

Abstract

Atherosclerosis is the primary pathological basis of cardiovascular diseases, with macrophage dysfunction, lipid accumulation, and oxidative stress driving plaque formation and progression. Ferroptosis, an iron-dependent form of regulated cell death characterized by lipid peroxidation, has recently emerged as a pivotal mechanism influencing atherosclerosis. Heme oxygenase-1 (HO-1), a key regulator of heme catabolism and iron homeostasis, exerts dual roles in this process: moderate HO-1 activity confers cytoprotection through antioxidant effects, whereas excessive HO-1 expression promotes intracellular iron accumulation, oxidative stress, and ferroptotic cell death. In macrophages, HO-1 mediates both classical ferroptosis pathways via glutathione peroxidase 4 (GPX4) regulation and non-classical, erythrophagocytosis-induced ferroptosis, contributing to plaque instability. This review systematically examines the molecular mechanisms underlying HO-1-induced ferroptosis in atherosclerosis, emphasizing its interplay with iron metabolism, oxidative stress, and macrophage function. Understanding the context-dependent effects of HO-1 provides novel insights into the regulation of vascular cell fate and plaque stability, highlighting potential therapeutic targets for the prevention and treatment of atherosclerotic cardiovascular diseases.

Keywords: ROS; atherosclerosis; ferroptosis; heme oxygenase-1; oxidative stress.

Publication types

  • Review

MeSH terms

  • Animals
  • Atherosclerosis* / etiology
  • Atherosclerosis* / metabolism
  • Atherosclerosis* / pathology
  • Ferroptosis*
  • Heme Oxygenase-1* / metabolism
  • Humans
  • Iron / metabolism
  • Lipid Peroxidation
  • Macrophages / immunology
  • Macrophages / metabolism
  • Oxidative Stress
  • Plaque, Atherosclerotic

Substances

  • Heme Oxygenase-1
  • Iron