Dynamically responsive hydrogel with mechanical stimulation enhances diabetic wound healing via activation of Piezo1-mediated efferocytosis

Bioact Mater. 2026 Feb 4:60:607-625. doi: 10.1016/j.bioactmat.2026.01.021. eCollection 2026 Jun.

Abstract

Correcting the disordered metabolism and achieving dynamic, comprehensive management of chronic diabetic wounds remains a significant challenge. This study presents a double-network dynamic hydrogel exhibiting long-term anti-inflammatory, antioxidant properties, and tunable mechanical strength. The hydrogel is primarily composed of modified chitosan, hyaluronic acid, sodium alginate, and ZnO2/Fe3+ nanoparticles. The incorporated ZnO2/Fe3+ nanoparticles enable microenvironmental regulation by responding to H+ or reactive oxygen species (ROS), while the released Fe3+ ions drive hydrogel network reconstruction, thereby enhancing mechanical properties. In vitro studies demonstrate the hydrogel's efficacy in efficiently scavenging ROS and enhancing Piezo1-mediated macrophage efferocytosis through cell-matrix interactions, accelerating macrophage polarization towards the M2 phenotype and resolving inflammation. In vivo experiments further confirm that the CHS@ZnO2/Fe3+ hydrogel significantly promotes re-epithelialization. Mechanical stimulation provided by the hydrogel recruited abundant fibroblasts and endothelial cells to the wound site, facilitating collagen deposition and angiogenesis. This novel hydrogel dressing, combining mechanical and biochemical dual-regulation, provides an advanced therapeutic strategy for the efficient repair of diabetic chronic wounds.

Keywords: Diabetic wounds; Dynamic mechanical stimulation; Efferocytosis; Piezo1.