Spatial Profiling of Germinal Centers in Mouse Secondary Lymphoid Organs Using MACSima Imaging Cyclic Staining Technology

Isabel San Martín Molina; Hanneke Okkenhaug; Simon Walker; Michelle Linterman; Edith Marcial-Juárez

doi:10.1002/eji.70157

Spatial Profiling of Germinal Centers in Mouse Secondary Lymphoid Organs Using MACSima Imaging Cyclic Staining Technology

Eur J Immunol. 2026 Feb;56(2):e70157. doi: 10.1002/eji.70157.

Authors

Isabel San Martín Molina¹, Hanneke Okkenhaug¹, Simon Walker¹, Michelle Linterman^{2

3}, Edith Marcial-Juárez²

Affiliations

¹ Imaging Facility, Babraham Institute, Babraham Research Campus, Cambridge, UK.
² Immunology Program, Babraham Institute, Babraham Research Campus, Cambridge, UK.
³ Malaghan Institute of Medical Research, Wellington, New Zealand.

Abstract

We developed a 50-plex imaging protocol using the MACSima platform to characterize the microarchitecture of germinal centers in secondary lymphoid tissues. This workflow combines tissue processing, automated cyclic imaging, image preprocessing, and a new analysis method that detects morphological changes in irregularly shaped stromal cells.

Keywords: B cells; MACSima imaging cyclic staining; T follicular helper cells; follicular dendritic cells; germinal centers.

MeSH terms

Animals
Germinal Center* / cytology
Germinal Center* / immunology
Image Processing, Computer-Assisted* / methods
Lymphoid Tissue* / immunology
Mice
Mice, Inbred C57BL
Staining and Labeling / methods

Abstract

MeSH terms

Grants and funding