Senise Red Pepper (Capsicum annuum L.) Wastes as Source of Rich Capsanthin Extracts by Supercritical CO2 Extraction

Molecules. 2026 Feb 19;31(4):715. doi: 10.3390/molecules31040715.

Abstract

The valorization of agro-industrial by-products represents a key strategy for promoting sustainable resource use and recovering high-value bioactive compounds. This study investigated the extraction of carotenoids from processing residues of Capsicum annuum L. cv Senise, a sweet pepper with Protected Geographical Indication (PGI) status, using supercritical CO2 extraction (SC-CO2). Experiments were conducted under nine pressure-temperature combinations (250-450 bar; 40-60 °C) for 60 min, and the results were compared with those obtained by conventional solvent extraction. The extraction yield ranged from 21.5 to 23.5 g/kgDW, with the highest total carotenoid content (386 mg/kgDW) achieved at 450 bar and 60 °C (SFE9), corresponding to a 70.2% recovery relative to the solvent method. HPLC analysis identified capsanthin (5.88 mg/g) as the predominant carotenoid in this extract, followed by zeaxanthin (2.16 mg/g), lutein (0.71 mg/g), β-cryptoxanthin (0.70 mg/g), β-carotene (0.58 mg/g) and capsorubin (0.38 mg/g). Overall, the results demonstrate that the green extraction of Senise pepper waste offers a sustainable approach to obtaining valuable natural pigments.

Keywords: Capsicum annuum L.; Senise peppers; capsanthin; carotenoids; supercritical CO2.

MeSH terms

  • Capsicum* / chemistry
  • Carbon Dioxide* / chemistry
  • Carotenoids / chemistry
  • Carotenoids / isolation & purification
  • Chromatography, High Pressure Liquid
  • Chromatography, Supercritical Fluid / methods
  • Plant Extracts* / chemistry
  • Plant Extracts* / isolation & purification
  • Xanthophylls / chemistry
  • Xanthophylls / isolation & purification

Substances

  • capsanthin
  • Carbon Dioxide
  • Plant Extracts
  • Xanthophylls
  • Carotenoids