Monkeypox is a viral zoonosis belonging to the genus Orthopoxvirus. Until now, cases of monkeypox were mostly isolated to endemic areas, however, many cases that do not even mention travel to the countries of Central and West Africa have appeared throughout Europe in the past year. The virus manifests itself as an outbreak of skin lesions followed by a general infectious syndrome. The frequent occurrence of skin lesions concentrated around the anogenital region was also seen in patients belonging to the MSM (Men Who Have Sex with Men) population, who admitted risky sexual relations. In this paper, we have presented a 28-year-old man who came to the University Clinical Center of Vojvodina with skin lesions in the genital area and was diagnosed with monkeypox infection by PCR multiplex from the lesions. The main goal of this case report is to determine the way the virus is transmitted and its clinical manifestations, to show diagnostic methods, as well as possible prevention measures and treatment options.