Cortex-wide characterization of decision-making neural dynamics during spatial navigation

Abstract

Making decisions while moving through the environment requires extensive interactions among cerebral cortical regions. To assess the spatial and temporal cortical dynamics during freely-moving decision making, we recorded widefield Ca²⁺ neuronal activity in mice navigating an 8-maze task with two paradigms. The first required an alternating figure-8 pattern to receive a reward. The second involved a rule change that only rewarded left turns. Cortical state analysis identified common global patterns of cortex-wide activity. We show that cortical states are dependent on maze location and represent features of spatial decision making, including outcome (correct or incorrect), choice (left or right turns), and task paradigm (alternating or left-only). Also, sequences of anterior and posterior propagating states occur throughout the maze. Anterior propagation is consistent with transforming sensory information from visual and parietal regions to anterior motor cortices to complete the task. Posterior propagation is consistent with top-down control from the secondary motor cortex for reward processing and internal feedback to guide future behavior.