Pharmacological and metabolic effects of Bifidobacterium triple viable capsules and Five-animal Play in patients with impaired glucose tolerance

Pak J Pharm Sci. 2026 May;39(5):1242-1253. doi: 10.36721/PJPS.2026.39.5.REG.15722.1.

Abstract

Background: Impaired glucose tolerance (IGT) is a reversible pre-diabetic condition characterized by early disruptions in insulin resistance and glucose control. Wingless-type MMTV integration site family member 5a/ secreted frizzled-related protein 5 (Wnt5a/Sfrp5) signaling and GLP-1 modulation have been identified as possible treatment approaches.

Objective: The effects of low-intensity aerobic activity, Huatuo Five-animal Play exercise and bifidobacterium triple viable capsules on glucose-lipid metabolism and GLP-1/Wnt5a/Sfrp5 signaling were assessed in this randomized, parallel-group clinical trial involving ninety persons with IGT.

Methods: The designated intervention was given to participants for four weeks. Before and during therapy, measurements were taken of fasting blood glucose (FBG), 2 h post-load glucose (2hFBG), glycosylated hemoglobin (HbA1c), fasting insulin, homeostasis model assessment of insulin resistance (HOMA-IR), serum lipids and circulating GLP-1, Wnt5a and Sfrp5.

Results: All treatments improved lipid indices, characterized by reductions in total cholesterol (TC), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C), along with an increase in high-density lipoprotein cholesterol (HDL-C) and significantly lowered FBG, 2hFBG, HbA1c, fasting insulin and HOMA-IR (P<0.05). Compared with aerobic exercise, Five-animal Play generated higher decreases in FBG, 2hFBG and HOMA-IR and a more significant decline in Wnt5a (P<0.05). Outcomes in the Five-animal Play group were comparable to the probiotic group. There were no negative responses or toxicities to the kidneys or liver.

Conclusion: Huatuo Five-animal Play may offer a safe, mechanism-based non-pharmacological approach for early metabolic intervention in pre-diabetes.

Keywords: Glucose metabolism; Five-animal Play exercise; Impaired glucose tolerance; Traditional Chinese medicine exercise therapy.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Adult
  • Animals
  • Bifidobacterium*
  • Blood Glucose* / metabolism
  • Capsules
  • Exercise Therapy* / methods
  • Female
  • Glucagon-Like Peptide 1 / blood
  • Glucagon-Like Peptide 1 / metabolism
  • Glucose Intolerance* / blood
  • Glucose Intolerance* / metabolism
  • Glucose Intolerance* / therapy
  • Glycated Hemoglobin / metabolism
  • Humans
  • Insulin / blood
  • Insulin Resistance
  • Lipids / blood
  • Male
  • Middle Aged
  • Probiotics* / administration & dosage
  • Probiotics* / therapeutic use
  • Treatment Outcome
  • Wnt-5a Protein / blood
  • Wnt-5a Protein / metabolism

Substances

  • Blood Glucose
  • Glucagon-Like Peptide 1
  • Insulin
  • Capsules
  • Wnt-5a Protein
  • Glycated Hemoglobin
  • Lipids