Sucrase and lactase synthesis in suckling rat intestine in response to substrate administration

Biol Neonate. 1978;33(1-2):100-5. doi: 10.1159/000241057.

Abstract

The intestinal brush border disaccharidases separated by gel electrophoresis were studied after oral administration of a high sucrose or lactose diet to 11-day-old suckling rats during 3 days. Some modifications of the brush border protein and eyzyme patterns could be attributed to the effect of the basic diet: increase of glucoamylase, appearance of a weak sucrase activity and of a second molecular form of maltase. However, the specific action of a given disaccharide on the synthesis of the corresponding hydrolytic enzyme could be clearly demonstrated. Indeed, the electrophoretic pattern after sucrose or lactose feeding showed a marked increase of the protein bands corresponding to sucrase-isomaltase or lactase activities.

MeSH terms

  • Administration, Oral
  • Animals
  • Animals, Newborn*
  • Electrophoresis, Polyacrylamide Gel
  • Female
  • Galactosidases / biosynthesis*
  • Intestinal Mucosa / enzymology*
  • Intestine, Small / enzymology
  • Lactose / administration & dosage
  • Pregnancy
  • Rats
  • Sucrase / biosynthesis*
  • Sucrose / administration & dosage
  • beta-Galactosidase / biosynthesis*

Substances

  • Sucrose
  • Galactosidases
  • beta-Galactosidase
  • Sucrase
  • Lactose