Best Practices in O-GlcNAcylation: A Survey-Based Consensus Among Researchers

Abstract

O-GlcNAcylation is a dynamic and reversible post-translational modification contributing to various cellular processes and diseases. Despite its growing relevance, standardized methodologies and reproducibility remain challenges in the field. To address this, we conducted an international survey among researchers active in O-GlcNAc research to identify current practices, bottlenecks, and consensus recommendations. Here, we present the key findings of O-GlcNAc researchers worldwide. The data reveal variability in detection techniques, sample preparation, and data interpretation, underscoring the need for harmonized protocols. Based on these insights, we propose a set of best practices to improve rigor and reproducibility in O-GlcNAc research.

Keywords: O-GlcNAc; best practices; community; methods; reproducibility.