A total of 274 strains of Micrococcus indolicus, 211 of which had been isolated from cases of summermastitis, 13 from other cases of mastitis in cattle, 15 from other suppurative lesions in cattle, 13 from insects, 13 from the vagina and interdigital skin of clinically healthy cows, and 9 from various suppurative lesions in swine, were studied and compared with 4 strains of anaerobic cocci of human origin, presumably representing at least 3 species closely related to Mi. indolicus: Staph. asaccharolyticus Distaso, Staph. aerogenes Schotmüller, and Staph. anaerobius Jungano.
The growth characteristics of Mi. indolicus are described, and the most important biochemical criteria for its identification stated briefly (Table 1).
By double diffusion-in-gel analysis 217 strains of Mi. indolicus could be divided in 6 antigenic types, designated A, B, C, D, E, and F (Figures 2, 3, 4, and 5, Tables 2, 3, 4, and 6).
By the complement fixation technique no difference could be demonstrated between the 6 types (Table 5).
Strains isolated from healthy cattle or from insects all belonged to antigenic types commonly found in summermastitis.
Three of 9 porcine strains belonged to a type (F) hitherto not found in cattle.
The 4 human strains of anaerobic cocci showed no antigenic relation to Mi. indolicus (Table 5) and differed from it in growth characteristics and in biochemical properties (Table 1).
It is concluded that, as suggested by Christiansen (1934) Micrococcus indolicus should be classified as a species of its own and not as a variant of Staphylococcus asaccharolyticus Distaso (Prévot et al. 1967).
Undersøgelsen omfatter ialt 274 stammer af Micrococcus indolicus (heraf 211 isoleret fra sommermastitis, 13 fra andre mastiter hos kvæg, 15 fra andre suppurative lidelser hos kvæg, 13 fra insekter, 13 fra vagina eller klovspalte hos klinisk sunde køer og 9 fra forskellige suppurative lidelser hos svin) samt 4 stammer af anaerobe kokker af human oprindelse, som formentlig repræsenterer mindst 3 Mi. indolicus nærtstående arter: Staph. asaccharolyticus Distaso, Staph. aerogenes Schottmüller og Staph. anaerobius Jungano.
Mi. indolicus’ kulturelle egenskaber samt de vigtigste biokemiske kriterier for dens identifikation beskrives (tabel 1).
Ved gel diffusions analyse af 217 Mi. indolicus stammer påvistes 6 antigene typer: A, B, G, D, E og F (fig. 2, 3, 4 og 5, tabel 2, 3, 4 og 6), medens der ved komplementbindingsprøve ikke fandtes antigene forskelle mellem de 6 typer (tabel 5).
Stammer isoleret fra klinisk sunde dyr og fra insekter var alle af en af de 4 typer, som hyppigst påvistes blandt stammer isoleret fra sommermastitis.
Tre af 9 porcine stammer tilhørte en type (F), som endnu ikke er påvist hos kvæg.
De 4 humane stammer var antigent forskellige fra Mi. indolicus (tabel 5) og afveg endvidere i biokemiske og kulturelle egenskaber (tabel 1).
Det konkluderes, som formodet af Christiansen (1934), at Micrococcus indolicus Christiansen bør klassificeres som en selvstændig species og ikke som en variant af Staphylococcus asaccharolyticus Distaso (Prévot et al. 1967).