A crRNA/Cas12a complex-driven rapid and visual detection method for four porcine diarrhea viruses

Lei Lei; Lei Tan; Yingjie Chen; Xiaoye Peng; Yuge Wang; Fan Liao; Youming Yuan; Yingyi Chen; Kaixin Wang; Xiaojiu Huang; Yang Zhan; Naidong Wang; Deyong Duan; Yi Yang; Aibing Wang

doi:10.1186/s12917-026-05351-9

A crRNA/Cas12a complex-driven rapid and visual detection method for four porcine diarrhea viruses

BMC Vet Res. 2026 Apr 18. doi: 10.1186/s12917-026-05351-9. Online ahead of print.

Authors

Lei Lei^#¹, Lei Tan^#², Yingjie Chen¹, Xiaoye Peng¹, Yuge Wang¹, Fan Liao¹, Youming Yuan³, Yingyi Chen¹, Kaixin Wang¹, Xiaojiu Huang¹, Yang Zhan¹, Naidong Wang¹, Deyong Duan¹, Yi Yang⁴, Aibing Wang⁵

Affiliations

¹ College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China.
² College of Animal Science and Technology, Yangtze University, Jingzhou, Hubei, 434025, China.
³ College of Food Science & Nutritional Engineering, China Agricultural University, Beijing, 100083, China.
⁴ College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China. yiyang@hunau.edu.cn.
⁵ College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China. bingaiwang@hunau.edu.cn.

^# Contributed equally.

PMID: 42001168
DOI: 10.1186/s12917-026-05351-9

No abstract available

Keywords: Multiple LAMP; Porcine delta-coronavirus (PDCoV); Porcine epidemic diarrhea virus (PEDV); Porcine rotavirus-A (PoRV) G9; Transmissible gastroenteritis virus (TGEV); crRNA/Cas12a complex.

Grants and funding

2021NK1030/Research and development of protein particle vaccines for African Swine Fever and other major viral diseases