A 4-dimensional spatial gene expression atlas of Hordeum vulgare (barley) grain development and germination was generated using spatial transcriptomic analysis of serial sections to reconstruct transcript abundance in 3 physical dimensions and with temporal kinetics. We investigated the subtissue localizations of specific biological activities, using energy biology as an example, including genes encoding proteins involved in starch synthesis and degradation, sugar transport, and mitochondrial and chloroplast activity. This atlas revealed different patterns in gene expression across tissues and developmental stages. Heterogeneity in gene expression was observed between clusters, within domains of the individual clusters, across 2-dimensional (xy, 55-μm resolution) and 3-dimensional (xyz, 8-μm resolution in z-plane) axes. Yet, other genes, including typical housekeeping genes such as Actin, Tubulin, and others, display homogeneous expression patterns. Expression of several genes matched previous gene-specific studies in different barley varieties verifying the robustness of the approach and indicating that patterns of gene expression are conserved at least for some categories of genes between varieties. Trajectory analysis of aleurone tissue spanning from early development to the completion of germination, provided a comprehensive roadmap of tissue development in terms of processes and identified transcription factors with spatial specificity that play roles in seed development and germination. A public visualization browser is available to view 2- and 3-dimensional transcription abundance profiles at https://barley-4d.latrobe.edu.au/or https://barley-4d-gene-atlas.hutton.ac.uk/.
© The Author(s) 2026. Published by Oxford University Press on behalf of American Society of Plant Biologists.