Human Pancreatic Cancer Organoids

Aina He; Xiaolin Lin; Xiaozhe Qian; Jiasheng Mu; Zhonghua Tao; Jin Gu; Yonggang Wang; Ting Han; Hongling Zhao; Yalong Wang; Jian Zhang; Zunqiang Zhou; Ligang Xing; Changchun Zhou; Dongyuan Zhu; Zengjun Liu; Gang Chen; Qinghe Zhou; Xiaoguang Wang; Shasha Zhao; Xiao Liu; Dongyan Cao; Bin Yu; Zebing Liu; Jiabei Wang; Gengming Niu; Guiying Wei; Maorong Chen; Weiping Wang; Xiaonan Kang; Junmei Zhou; Shuai Gong; Ying Xie; Jianming Zhang; Xianming Kong; Chunyan Dong; Yanmei Zou; Lina Tang; Aijin Ma; Hua Xiong; Xuemei Liu; Tongbiao Zhao; Jie Hao; Xiuying Xiao; Dongxi Xiang

doi:10.1111/cpr.70210

Human Pancreatic Cancer Organoids

Cell Prolif. 2026 Apr 23:e70210. doi: 10.1111/cpr.70210. Online ahead of print.

Authors

Aina He¹, Xiaolin Lin², Xiaozhe Qian³, Jiasheng Mu⁴, Zhonghua Tao⁵, Jin Gu⁶, Yonggang Wang¹, Ting Han³, Hongling Zhao^{7

8}, Yalong Wang⁹, Jian Zhang¹⁰, Zunqiang Zhou¹¹, Ligang Xing¹², Changchun Zhou¹³, Dongyuan Zhu¹⁴, Zengjun Liu¹⁴, Gang Chen¹⁵, Qinghe Zhou¹⁵, Xiaoguang Wang¹⁶, Shasha Zhao¹⁷, Xiao Liu¹⁷, Dongyan Cao¹⁷, Bin Yu¹⁷, Zebing Liu¹⁸, Jiabei Wang¹⁹, Gengming Niu²⁰, Guiying Wei²⁰, Maorong Chen²¹, Weiping Wang²², Xiaonan Kang²³, Junmei Zhou²⁴, Shuai Gong²⁵, Ying Xie²⁶, Jianming Zhang²⁷, Xianming Kong²⁸, Chunyan Dong²⁹, Yanmei Zou³⁰, Lina Tang¹, Aijin Ma^{7

31}, Hua Xiong³⁰, Xuemei Liu³², Tongbiao Zhao^{7

8

33}, Jie Hao^{7

34}, Xiuying Xiao², Dongxi Xiang^{17

35

36}

Affiliations

¹ Department of Oncology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
² Department of Oncology, Renji Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.
³ Department of Thoracic Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
⁴ Department of General Surgery, Xinhua Hospital, Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai, China.
⁵ Department of Medical Oncology, Fudan University Shanghai Cancer Center, Department of Oncology, Shanghai Medical College, State Key Laboratory of Genetic Engineering, School of Life Sciences, Human Phenome Institute, Fudan University, Shanghai, China.
⁶ Department of Hepatobiliary and Pancreatic Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, China.
⁷ Beijing Institute for Stem Cell and Regeneration Medicine, Beijing, China.
⁸ Standard Committee, Chinese Society for Cell Biology, Shanghai, China.
⁹ The MOE Basic Research and Innovation Center for the Targeted Therapeutics of Solid Tumors, School of Basic Medical Sciences, Institute of Biomedical Innovation, Jiangxi Medical College, Nanchang University, Nanchang, China.
¹⁰ Department of Medical Oncology, Fudan University Shanghai Cancer Center, Shanghai, China.
¹¹ Department of Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
¹² Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University, Shandong Academy of Medical Science, Jinan, China.
¹³ Biobank, Cancer Research Center, Shandong Cancer Hospital and Institute, Shandong First Medical University, Shandong Academy of Medical Sciences, Jinan, China.
¹⁴ Rare Tumors Department, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, China.
¹⁵ Jiaxing Key Laboratory of Basic Research and Clinical Translation on Orthopedic Biomaterials, Department of Orthopaedics, The Second Affiliated Hospital of Jiaxing University, Jiaxing, China.
¹⁶ Department of Hepatobiliary Surgery, Affiliated Hospital of Jiaxing University, Jiaxing, China.
¹⁷ State Key Laboratory of Systems Medicine for Cancer, Shanghai Cancer Institute, Shanghai Key Laboratory of Cancer System Regulation and Clinical Translation, Jiading Branch, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
¹⁸ Department of Pathology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
¹⁹ Department of Hepatobiliary Surgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Anhui, China.
²⁰ Shanghai OneTar Biomedicine, Shanghai, China.
²¹ Center for Life Science, Yunnan Key Laboratory of Cell Metabolism and Diseases, School of Life Sciences, Yunnan University, Kunming, China.
²² Department of Pharmacology and Pharmacy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.
²³ Department of Biobank, Renji Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.
²⁴ Department of Central Laboratory, Shanghai Children's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
²⁵ Division of Gastroenterology and Hepatology, Renji Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.
²⁶ Life Sciences Institute, Guangxi Medical University, Nanning, China.
²⁷ Institute of Translational Medicine, Zhangjiang Institute for Advanced Study, Shanghai Jiao Tong University, Shanghai, China.
²⁸ Collaborative Research Center for Biomedicines, Shanghai University of Medicine and Health Sciences, Shanghai, China.
²⁹ Department of Oncology, East Hospital Affiliated to Tongji University, Tongji University School of Medicine, Shanghai, China.
³⁰ Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
³¹ Beijing Technology and Business University, Beijing, China.
³² Department of Gastroenterology, Digestive Disease Hospital, Affiliated Hospital of Zunyi Medical University, Zunyi, China.
³³ Key Laboratory of Organ Regeneration and Reconstruction, State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
³⁴ National Stem Cell Resource Center, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
³⁵ Key Laboratory of Early Prevention and Treatment for Regional High Frequency Tumor (Guangxi Medical University), Ministry of Education, Nanning, China.
³⁶ Jiaxing Organoid Center, Jiaxing, Zhejiang, China.

Abstract

This guideline establishes a comprehensive framework for the application of patient-derived pancreatic cancer organoids. It outlines the stringent technical requirements and testing methods necessary to ensure high fidelity to the original tumor tissue, including morphological assessment, pathological biomarker expression (e.g., CK19, CK7), and genetic concordance (e.g., NRG1, KRAS). By standardizing protocols for in vitro culture, microbiological safety, and STR authentication, this consensus aims to ensure the reproducibility, safety, and stability of organoid models, thereby accelerating their integration into basic research, drug discovery, and precision medicine.

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Editorial

Abstract

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