Determination of ATP Content in Cells

Boqiang Fu; Wenfeng Huang; Yingying Liu; Lei Wang; Yuan Liu; Jiani Cao; Wenjuan Duan; Aijin Ma; Hongling Zhao; Shuaishuai Niu; Shijun Hu; Qiyuan Li; Yong Zhang; Yaojin Peng; Xiaoyou Yu; Junying Yu; Jun Wei; Yu Zhang; Guoqiang Hua; Xin Liu; Changlin Wang; Tao Na; Yang Zhao; Jiaxi Zhou; Peng Xiang; Zhihong Wu; Qubo Chen; Peijun Zhai; Hengjun Gao; Jie Hao; Tongbiao Zhao; Jing Wang

doi:10.1111/cpr.70184

Determination of ATP Content in Cells

Cell Prolif. 2026 Apr 27:e70184. doi: 10.1111/cpr.70184. Online ahead of print.

Authors

Boqiang Fu^{1

2}, Wenfeng Huang¹, Yingying Liu¹, Lei Wang^{2

3}, Yuan Liu⁴, Jiani Cao^{5

6}, Wenjuan Duan⁷, Aijin Ma^{2

8}, Hongling Zhao^{2

9}, Shuaishuai Niu^{2

3}, Shijun Hu^{2

10}, Qiyuan Li¹¹, Yong Zhang¹², Yaojin Peng^{2

3

9}, Xiaoyou Yu^{2

3}, Junying Yu¹³, Jun Wei⁵, Yu Zhang⁵, Guoqiang Hua¹⁴, Xin Liu⁷, Changlin Wang¹⁵, Tao Na⁶, Yang Zhao¹⁶, Jiaxi Zhou¹⁷, Peng Xiang¹⁸, Zhihong Wu¹⁹, Qubo Chen²⁰, Peijun Zhai²¹, Hengjun Gao²², Jie Hao^{2

3

9}, Tongbiao Zhao^{2

3

9

23}, Jing Wang¹

Affiliations

¹ National Institute of Metrology, Beijing, China.
² Standard Committee, Chinese Society for Cell Biology, Shanghai, China.
³ Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
⁴ Tsinghua University, Beijing, China.
⁵ Zephyrm Biotechnologies Co., Ltd., Beijing, China.
⁶ National Institutes for Food and Drug Control, Beijing, China.
⁷ Promega (Beijing) Biotech Co., Ltd., Beijing, China.
⁸ Beijing Technology and Business University, Beijing, China.
⁹ Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, China.
¹⁰ Soochow University, Suzhou, China.
¹¹ Shenzhen Medical Academy of Research and Translation, Shenzhen, China.
¹² Shenzhen HHLIFE Co., Ltd., Shenzhen, China.
¹³ Nuwacell Biotechnologies Co., Ltd., Hefei, China.
¹⁴ D1 Medical Technology, Shanghai, China.
¹⁵ China National Institute of Standardization, Beijing, China.
¹⁶ Peking University, Beijing, China.
¹⁷ Institute of Radiation Medicine Chinese Academy of Medical Sciences, Tianjin, China.
¹⁸ Sun Yat-sen University, Guangzhou, China.
¹⁹ Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China.
²⁰ The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, China.
²¹ China National Accreditation Service for Conformity Assessment (CNAS), Beijing, China.
²² Shanghai National Engineering Research Center of Biochip, Shanghai Engineering Center for Molecular Medicine, Shanghai, China.
²³ University of Chinese Academy of Sciences, Beijing, China.

PMID: 42044874
DOI: 10.1111/cpr.70184

Abstract

The overlap HPLC chromatograms of ATP standard (red) and ATP in cells (blue). Poroshell 120 EC-C18 (3 × 150 mm, 2.7 μm), 25°C, pH 6.8 buffer solution of 0.05 mol/L KH₂PO₄ - 0.05 mol/L K₂HPO₄ (V:V = 1:1) as mobile phase, 0.6 mL/min, 254 nm.

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