A simple graphical method for determining the inhibition constants of mixed, uncompetitive and non-competitive inhibitors

Biochem J. 1974 Jan;137(1):143-4. doi: 10.1042/bj1370143.

Abstract

A new method is described for plotting kinetic results for inhibited enzyme-catalysed reactions. It provides a simple way of determining the inhibition constant, K'(i), of an uncompetitive, mixed or non-competitive inhibitor.

Publication types

  • Comparative Study

MeSH terms

  • Binding Sites
  • Binding, Competitive
  • Enzyme Inhibitors*
  • Evaluation Studies as Topic
  • Kinetics*
  • Mathematics
  • Methods
  • Models, Chemical
  • Protein Binding

Substances

  • Enzyme Inhibitors