Combined cyclosporine A and urolithin A therapy ameliorates murine lupus nephritis

J Immunol. 2026 Apr 15;215(4):vkag087. doi: 10.1093/jimmun/vkag087.

Abstract

Monotherapies for lupus nephritis (LN) often fail to fully control the disease's hallmark, renal inflammation and immune complex deposition. This study investigates a novel combination therapeutic strategy using urolithin A (UA), a multifaceted anti-inflammatory and antioxidant agent, with cyclosporine A (CsA), an established immunosuppressant. The combination therapy's superior efficacy is evidenced by a robust reduction in immunoglobulin G (IgG) anti-dsDNA levels, with markedly improved renal function. The treatment also effectively mitigated immune complex deposition and multiple inflammatory chemokines, including I309, IL-16, and MIP-3. This alleviated kidney damage and suppressed lymphocyte infiltration. We found that CsA alone was ineffective across the analyzed markers, while UA alone produced only a modest effect, highlighting the complementary action of their combination. These findings underscore the potent anti-inflammatory and antioxidant properties of UA and suggest that combining it with CsA offers a more robust strategy for controlling inflammation and preserving renal integrity in LN. Given the FDA-approved status of CsA and UA's "generally recognized as safe" (GRAS) classification, this combination therapy presents a promising and practical clinical pathway for the treatment of lupus nephritis.

Keywords: combined therapy; cyclosporine A; lupus nephritis; pro-inflammatory pathways; urolithin A.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents* / therapeutic use
  • Antibodies, Antinuclear / blood
  • Antigen-Antibody Complex / immunology
  • Antigen-Antibody Complex / metabolism
  • Antioxidants / therapeutic use
  • Coumarins* / administration & dosage
  • Coumarins* / pharmacology
  • Coumarins* / therapeutic use
  • Cyclosporine* / pharmacology
  • Cyclosporine* / therapeutic use
  • Disease Models, Animal
  • Drug Therapy, Combination
  • Female
  • Immunoglobulin G / blood
  • Immunoglobulin G / immunology
  • Immunosuppressive Agents* / pharmacology
  • Immunosuppressive Agents* / therapeutic use
  • Kidney / drug effects
  • Kidney / immunology
  • Kidney / pathology
  • Lupus Nephritis* / drug therapy
  • Lupus Nephritis* / immunology
  • Lupus Nephritis* / pathology
  • Mice

Substances

  • Cyclosporine
  • Immunosuppressive Agents
  • Coumarins
  • 3,8-dihydroxy-6H-dibenzo(b,d)pyran-6-one
  • Anti-Inflammatory Agents
  • Antioxidants
  • Immunoglobulin G
  • Antibodies, Antinuclear
  • Antigen-Antibody Complex