Phage Bouclier has a 19,912bp genome with 29 protein coding genes and infects Arthrobacter globiformis B-2979. Transmission electron microscopy visualizes Bouclier as a podovirus. This is confirmed by identification of genes encoding structural proteins found in other podoviriruses. The portal, upper collar, tail knob, and major capsid proteins can be modeled using AlphaFold3 into macromolecular structures. These structural components have high confidence matches in ChimeraX to Bacillus phage Φ29 that has been resolved by cryoEM. Bouclier contains a defined lysis cassette including two genes encoding Lysin A proteins and three putative transmembrane proteins that may be involved in host lysis.
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