Purification and amino acid analysis of an active site peptide from thymidylate synthetase containing covalently bound 5-fluoro-2'-deoxyuridylate and methylenetetrahydrofolate

Biochem Biophys Res Commun. 1974 Apr 8;57(3):689-95. doi: 10.1016/0006-291x(74)90601-9.
No abstract available

MeSH terms

  • Amino Acid Sequence
  • Amino Acids / analysis
  • Binding Sites
  • Carbon Radioisotopes
  • Chromatography, Gel
  • Chromatography, Ion Exchange
  • Drug Resistance, Microbial
  • Electrophoresis, Paper
  • Floxuridine*
  • Lactobacillus casei / drug effects
  • Lactobacillus casei / enzymology
  • Methotrexate / pharmacology
  • Methyltransferases*
  • Peptides
  • Pronase
  • Protein Binding
  • Spectrometry, Fluorescence
  • Spectrophotometry, Ultraviolet
  • Tetrahydrofolates*
  • Thymidylate Synthase / isolation & purification
  • Tritium
  • Uracil Nucleotides*

Substances

  • Amino Acids
  • Carbon Radioisotopes
  • Peptides
  • Tetrahydrofolates
  • Uracil Nucleotides
  • Floxuridine
  • Tritium
  • Methyltransferases
  • Thymidylate Synthase
  • Pronase
  • Methotrexate