Effects of an antibody to a highly purified Na+, K+-ATPase from canine renal medulla: separation of the "holoenzyme antibody" into catalytic and cardiac glycoside receptor-specific components

Proc Natl Acad Sci U S A. 1974 Jun;71(6):2449-52. doi: 10.1073/pnas.71.6.2449.

Abstract

An antiserum was prepared against a highly purified Na(+), K(+)-adenosine triphosphatase (ATP phosphohydrolase, EC 3.6.1.3). Purification and fractionation yielded two globulin components, one of which appears specific for a digitalis glycoside receptor site or binding conformation and the other for a catalytic site or conformation.

MeSH terms

  • Adenosine Triphosphatases / metabolism*
  • Animals
  • Antibodies / isolation & purification*
  • Antigen-Antibody Complex
  • Binding Sites
  • Binding Sites, Antibody*
  • Dogs
  • Immune Sera
  • Kidney / enzymology*
  • Kidney Medulla / enzymology
  • Ouabain / metabolism*
  • Potassium / metabolism
  • Protein Binding
  • Rabbits / immunology
  • Sodium / metabolism
  • Tritium

Substances

  • Antibodies
  • Antigen-Antibody Complex
  • Immune Sera
  • Tritium
  • Ouabain
  • Sodium
  • Adenosine Triphosphatases
  • Potassium