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, 254 (8), 2850-8

Primary Structure of a Human IgA1 Immunoglobulin. II. Isolation, Composition, and Amino Acid Sequence of the Tryptic Peptides of the Whole alpha1 Chain and Its Cyanogen Bromide Fragments

  • PMID: 429322

Primary Structure of a Human IgA1 Immunoglobulin. II. Isolation, Composition, and Amino Acid Sequence of the Tryptic Peptides of the Whole alpha1 Chain and Its Cyanogen Bromide Fragments

T L Low et al. J Biol Chem.

Abstract

As part of the strategy for determining the covalent structure of a human IgA1 molecule (Bur), a tryptic digest was prepared of the reduced and carboxymethylated alpha1 heavy chain. In addition to the main experiment, tryptic peptides were prepared from the succinylated aminoethylated alpha1 chain and from fragments obtained by CNBr scission of the alpha1 chain. Complete recovery of the peptides was impeded by the large size of some of the tryptic peptides and of the principal CNBr fragment, and difficulty in separating other glycopeptides. Twenty-eight tryptic peptides of the reduced and carboxymethylated alpha1 chain were purified and sequenced, accounting for more than 300 residues. Additional information was obtained by sequence analysis of trypudies described in this series of papers contributed to the complete sequence analysis of the alpha1 chain.

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