Abstract
Stocks of vesicular stomatitis virus free of defective interfering particles were produced by serial clonal isolation. High-multiplicity infections with these stocks led to no interference or formation of defective interfering particles. Defective interfering particles were generated by three successive passages at high multiplicity.
MeSH terms
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Agar
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Animals
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Centrifugation, Density Gradient
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Clone Cells
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Cricetinae
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Culture Techniques*
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Dactinomycin
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Female
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Ovary
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RNA, Viral / analysis
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RNA, Viral / biosynthesis
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Sucrose
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Tritium
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Uridine / metabolism
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Vesicular stomatitis Indiana virus / analysis
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Vesicular stomatitis Indiana virus / growth & development*
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Vesicular stomatitis Indiana virus / isolation & purification
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Vesicular stomatitis Indiana virus / metabolism
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Vesicular stomatitis Indiana virus / pathogenicity
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Viral Interference*
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Virus Cultivation
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Virus Replication
Substances
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RNA, Viral
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Tritium
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Dactinomycin
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Sucrose
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Agar
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Uridine