Abstract
The deoxyribonucleic acid (DNA) polymerase of Rous sarcoma virus synthesizes both single- and double-stranded DNA, utilizing the ribonucleic acid (RNA) of the viral genome as the initial template. Results of pulse-chase experiments indicate that the single-stranded DNA serves as unconserved template and precursor for the synthesis of double-stranded DNA. The latter reaction is apparently initiated in association with the viral RNA and may involve a partially double-stranded intermediate form.
MeSH terms
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Animals
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Avian Sarcoma Viruses / enzymology*
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Avian Sarcoma Viruses / growth & development
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Avian Sarcoma Viruses / metabolism
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Centrifugation, Density Gradient
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Centrifugation, Zonal
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Chick Embryo
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Culture Techniques
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DNA Nucleotidyltransferases / metabolism*
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DNA, Viral / biosynthesis*
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DNA, Viral / isolation & purification
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Fibroblasts
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Genetic Code
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Genetics, Microbial*
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Hydroxyapatites
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Models, Theoretical
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Nucleotides / metabolism
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RNA, Viral / isolation & purification
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Ribonucleases / pharmacology
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Sucrose
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Templates, Genetic
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Tritium
Substances
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DNA, Viral
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Hydroxyapatites
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Nucleotides
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RNA, Viral
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Tritium
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Sucrose
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DNA Nucleotidyltransferases
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Ribonucleases