Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus influenzae

Proc Natl Acad Sci U S A. 1971 Dec;68(12):2913-7. doi: 10.1073/pnas.68.12.2913.

Abstract

A bacterial restriction endonuclease has been used to produce specific fragments of SV40 DNA. Digestion of DNA from plaque-purified stocks of SV40 with the restriction endonuclease from Hemophilus influenzae gave 11 fragments resolvable by polyacrylamide gel electrophoresis, eight of which were equimolar with the original DNA. The fragments ranged from about 6.5 x 10(5) to 7.4 x 10(4) daltons, as determined by electron microscopy, DNA content, or electrophoretic mobility.

MeSH terms

  • Acrylamides
  • Animals
  • Autoradiography
  • Biodegradation, Environmental
  • Carbon Isotopes
  • Cell Line
  • Centrifugation, Density Gradient
  • DNA, Viral / analysis
  • DNA, Viral / metabolism*
  • Deoxyribonucleases*
  • Detergents
  • Electrophoresis
  • Haemophilus influenzae / enzymology*
  • Haplorhini
  • Kidney
  • Microscopy, Electron
  • Molecular Weight
  • Phosphorus Isotopes
  • Simian virus 40 / metabolism*
  • Structure-Activity Relationship
  • Sulfates
  • Tritium

Substances

  • Acrylamides
  • Carbon Isotopes
  • DNA, Viral
  • Detergents
  • Phosphorus Isotopes
  • Sulfates
  • Tritium
  • Deoxyribonucleases