Differential isolation of microvillous and basolateral plasma membranes from intestinal mucosa: mutually exclusive distribution of digestive enzymes and ouabain-sensitive ATPase

Biochim Biophys Acta. 1972 Aug 9;274(2):336-47. doi: 10.1016/0005-2736(72)90181-2.
No abstract available

MeSH terms

  • Adenosine Triphosphatases / analysis*
  • Adenosine Triphosphatases / antagonists & inhibitors
  • Alkaline Phosphatase / analysis*
  • Animals
  • Cell Fractionation
  • Cell Membrane / drug effects
  • Cell Membrane / enzymology
  • Centrifugation, Density Gradient
  • Endopeptidases / analysis*
  • Female
  • Glucose-6-Phosphatase / analysis
  • Intestinal Mucosa / cytology
  • Intestinal Mucosa / drug effects
  • Intestinal Mucosa / enzymology*
  • Intestine, Small / cytology
  • Intestine, Small / drug effects
  • Intestine, Small / enzymology
  • Male
  • Mice
  • Microscopy, Electron
  • Ouabain / pharmacology
  • Oxidoreductases / analysis
  • Rats
  • Subcellular Fractions / enzymology
  • Sucrase / analysis*

Substances

  • Ouabain
  • Oxidoreductases
  • Alkaline Phosphatase
  • Glucose-6-Phosphatase
  • Sucrase
  • Endopeptidases
  • Adenosine Triphosphatases