In vitro polyoma DNA synthesis: studies on an early temperature-sensitive mutant

J Virol. 1974 Jan;13(1):241-3. doi: 10.1128/JVI.13.1.241-243.1974.

Abstract

The polyoma ts-a function was investigated by using an in vitro DNA-synthesizing system. A comparison of systems derived from ts25 (a ts-a group mutant)-and ts1260 (a late group mutant)-infected cells showed that the activation energies for DNA chain elongation and the mechanisms of discontinous growth were identical for both mutants.

MeSH terms

  • Animals
  • Cell Line
  • Centrifugation, Density Gradient
  • Cytosine Nucleotides / metabolism
  • DNA / biosynthesis
  • DNA Replication
  • DNA, Viral / biosynthesis*
  • Mice
  • Mutation*
  • Phosphorus Radioisotopes
  • Polyomavirus / growth & development
  • Polyomavirus / metabolism*
  • Temperature
  • Thymidine / metabolism
  • Time Factors
  • Tritium

Substances

  • Cytosine Nucleotides
  • DNA, Viral
  • Phosphorus Radioisotopes
  • Tritium
  • DNA
  • Thymidine