Purification of Clostridium Perfringens Phospholipase C (Alpha-Toxin) by Affinity Chromatography on Agarose-Linked Egg-Yolk Lipoprotein

Biochim Biophys Acta. 1974 May 10;351(1):155-71. doi: 10.1016/0005-2795(74)90074-9.

MeSH terms

  • Animals
  • Biological Assay
  • Chromatography, Affinity
  • Chromatography, Gel
  • Clostridium perfringens / enzymology*
  • Drug Stability
  • Egg Yolk
  • Electrophoresis, Polyacrylamide Gel
  • Female
  • Hemolysis
  • Hot Temperature
  • Hydrogen-Ion Concentration
  • Immunodiffusion
  • Isoelectric Focusing
  • Isoenzymes / isolation & purification
  • Lipoproteins
  • Mice
  • Molecular Weight
  • Phospholipases / isolation & purification*
  • Phospholipases / poisoning
  • Sheep
  • Spectrophotometry, Ultraviolet
  • Temperature
  • Time Factors
  • Toxins, Biological / isolation & purification*
  • Toxins, Biological / poisoning
  • Ultracentrifugation

Substances

  • Isoenzymes
  • Lipoproteins
  • Toxins, Biological
  • Phospholipases