Flow cytometry: a high-resolution instrument for everyone

Science. 1979 Apr 27;204(4391):403-4. doi: 10.1126/science.441727.

Abstract

A new flow configuration for flow cytometry has been devised in which a flat, laminar stream of water, containing the stained cells in a narrow sector, is formed on a microscope cover slip by a pressurized jet of water directed onto the glass at low angle. The stream of cells is viewed by means of a fluorescence microscope with incident illumination and a pulse photometer. Coupled to a multichannel pulse height analyzer, the instrument constitutes a stable and easy-to-operate flow cytometer with a resolution equal to or better than a coefficient of variance of 1.4 percent in measurements of cellular DNA.

MeSH terms

  • Animals
  • Cell Separation / instrumentation*
  • DNA / analysis*
  • Microscopy, Fluorescence / instrumentation
  • Rats
  • Thymus Gland / cytology

Substances

  • DNA