Abstract
Vinblastine treatment of microtubule protein or intact microtubules assembled in vitro produced bifilar rings and bifilar helices. Suspentsions of rings and helices were demonstrated to bind [(3)H]colchicine, a diagnostic property of microtubule protein. Macrotubules are suggested to consist of tightly coiled helices formed by longitudinal compacting of loosely coiled protofilament pair intermediates.
MeSH terms
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Animals
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Binding Sites
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Brain / ultrastructure
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Colchicine / metabolism
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In Vitro Techniques
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Macromolecular Substances
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Microscopy, Electron
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Microtubules / drug effects*
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Microtubules / ultrastructure
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Models, Structural
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Nerve Tissue Proteins* / metabolism
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Protein Binding
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Protein Conformation
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Swine
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Tritium
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Vinblastine / pharmacology*
Substances
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Macromolecular Substances
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Nerve Tissue Proteins
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Tritium
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Vinblastine
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Colchicine