Reconstitution into liposomes of glucose active transport from the rabbit renal proximal tubule. Characteristics of the system

Biochim Biophys Acta. 1979 May 17;553(2):295-306. doi: 10.1016/0005-2736(79)90233-5.

Abstract

This paper describes the characteristics of Na+ -dependent D-glucose transport into liposomes made from soybean phospholipids into which have been reconstituted detergent-solubilized components from the rabbit renal proximal tubular brush border membrane. Conditions for optimal and quantitative reconstitution of glucose carriers are defined. Na+ -dependent D-glucose uptake occurs via a saturable system with a Km of 0.125--0.135 mM, is responsive to the volume of the internal liposomal space, and shows 'overshoot' as seen in natural membranes. The rate of Na+-dependent D-glucose uptake and the magnitude of the 'overshoot" are proportional to the concentration of protein used in reconstitution.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Biological Transport, Active
  • Carrier Proteins / metabolism
  • Cell Membrane / metabolism
  • Cell-Free System
  • Glucose / metabolism*
  • Kidney Tubules, Proximal / metabolism*
  • Kidney Tubules, Proximal / ultrastructure
  • Kinetics
  • Liposomes
  • Membrane Proteins / analysis
  • Rabbits
  • Sonication
  • Water-Electrolyte Balance

Substances

  • Carrier Proteins
  • Liposomes
  • Membrane Proteins
  • Glucose