Studies on proteinases from Calotropis gigantea latex. I. Purification and some properties of two proteinases containing carbohydrate

Biochim Biophys Acta. 1979 May 10;568(1):111-9. doi: 10.1016/0005-2744(79)90278-x.

Abstract

Two proteinase containing carbohydrate, called calotropain-FI and calotropain-FII, were purified from Calotropis gigantea latex by CM-Sephadex C-50 chromatography. Both calotropain-FI and FII were found to be homogeneous by rechromatography on CM-Sephadex C-50, gel filtration on Sephadex G-100, electrophoresis on polyacrylamide gel and by N-terminal amino acid analysis. Some properties of these enzymes are reported.

MeSH terms

  • Amino Acids / analysis
  • Carbohydrates / analysis
  • Cysteine Endopeptidases
  • Endopeptidases / isolation & purification*
  • Endopeptidases / metabolism
  • Isoenzymes / isolation & purification
  • Isoenzymes / metabolism
  • Kinetics
  • Plants / enzymology*
  • Rubber*

Substances

  • Amino Acids
  • Carbohydrates
  • Isoenzymes
  • Rubber
  • Endopeptidases
  • Cysteine Endopeptidases
  • calotropain-FI
  • calotropain-FII