Quantitation of the tetramethyl-p-phenylenediamine oxidase reaction in Neisseria species

Appl Microbiol. 1974 Dec;28(6):1079-81.


The tetramethyl-p-phenylenediamine oxidase reaction commonly used in the Kovacs oxidase test was quantitatively estimated for various Neisseria species employing standardized resting cell suspensions. This genus of microorganisms exhibited very high tetramethyl-p-phenylenediamine oxidase rates comparable to that of Azotobacter and Pseudomonas, and this reaction was found to be a valid measurement for the respiratory capability possessed by this group of organisms.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Oxidoreductases / metabolism*
  • Fructose / metabolism
  • Glucose / metabolism
  • Lactose / metabolism
  • Neisseria / classification
  • Neisseria / enzymology*
  • Neisseria / metabolism
  • Oxidation-Reduction
  • Oxygen Consumption
  • Phenylenediamines
  • Species Specificity
  • Sucrose / metabolism


  • Phenylenediamines
  • Fructose
  • Sucrose
  • Amino Acid Oxidoreductases
  • Glucose
  • Lactose