Calf lens crystallin messenger RNA's contain polynucleotide sequences rich in adenylic acid

Nucleic Acids Res. 1974 Feb;1(2):245-55. doi: 10.1093/nar/1.2.245.

Abstract

Calf lens messenger fractions have been purified on poly(dT)-cellulose. The purified RNA's have been analyzed as nucleosides by a method which can be used on a micro-scale with highly reproduceble results. Both the 10S and 14S lens mRNA contain poly A sequences. The poly A content found in 14S mRNA cannot account for the excess nucleotides in this message.

MeSH terms

  • Adenosine Monophosphate
  • Animals
  • Base Sequence
  • Cattle
  • Cellulose
  • Centrifugation, Density Gradient
  • Centrifugation, Zonal
  • Chromatography, Affinity
  • Chromatography, Ion Exchange
  • Crystallins
  • Lens, Crystalline / analysis
  • Lens, Crystalline / cytology
  • Polynucleotides*
  • Polyribosomes / analysis
  • RNA, Messenger* / analysis
  • RNA, Messenger* / isolation & purification
  • Ribonucleotides / analysis
  • Sodium Dodecyl Sulfate
  • Spectrophotometry, Ultraviolet
  • Temperature
  • Thymidine
  • Time Factors

Substances

  • Crystallins
  • Polynucleotides
  • RNA, Messenger
  • Ribonucleotides
  • Sodium Dodecyl Sulfate
  • Adenosine Monophosphate
  • Cellulose
  • Thymidine