Abstract
Calf lens messenger fractions have been purified on poly(dT)-cellulose. The purified RNA's have been analyzed as nucleosides by a method which can be used on a micro-scale with highly reproduceble results. Both the 10S and 14S lens mRNA contain poly A sequences. The poly A content found in 14S mRNA cannot account for the excess nucleotides in this message.
MeSH terms
-
Adenosine Monophosphate
-
Animals
-
Base Sequence
-
Cattle
-
Cellulose
-
Centrifugation, Density Gradient
-
Centrifugation, Zonal
-
Chromatography, Affinity
-
Chromatography, Ion Exchange
-
Crystallins
-
Lens, Crystalline / analysis
-
Lens, Crystalline / cytology
-
Polynucleotides*
-
Polyribosomes / analysis
-
RNA, Messenger* / analysis
-
RNA, Messenger* / isolation & purification
-
Ribonucleotides / analysis
-
Sodium Dodecyl Sulfate
-
Spectrophotometry, Ultraviolet
-
Temperature
-
Thymidine
-
Time Factors
Substances
-
Crystallins
-
Polynucleotides
-
RNA, Messenger
-
Ribonucleotides
-
Sodium Dodecyl Sulfate
-
Adenosine Monophosphate
-
Cellulose
-
Thymidine