A new synthetic RNA-dependent DNA polymerase from human tissue culture cells (HeLa-fibroblast-synthetic oligonucleotides-template-purified enzymes)

Proc Natl Acad Sci U S A. 1972 Feb;69(2):452-5. doi: 10.1073/pnas.69.2.452.


Two DNA polymerases that can copy synthetic RNA polymers are present in human tissue culture cells. These enzymes which have each been purified about 500-fold, are present in both HeLa cells, which are derived from a cervical carcinoma, and in WI-38 cells, a normal diploid strain originating from human embryonic lung tissue. These synthetic RNA-dependent DNA polymerases are identified by their ability to copy efficiently the ribo strand of synthetic oligonucleotide-homopolymer complexes, and differ in this respect from the known DNA-dependent DNA polymerases found in HeLa cells. The template requirements of these new DNA polymerases resemble that of the RNA-dependent DNA polymerases of the RNA tumor-viruses.

MeSH terms

  • Cell Line
  • Cells, Cultured / analysis
  • Chromatography, DEAE-Cellulose
  • DNA / metabolism
  • DNA Nucleotidyltransferases / isolation & purification*
  • Deoxyribonucleotides
  • HeLa Cells / drug effects
  • HeLa Cells / enzymology*
  • HeLa Cells / metabolism
  • Humans
  • Manganese / pharmacology
  • Polynucleotides
  • Protein Binding
  • RNA / metabolism*
  • Templates, Genetic
  • Tritium


  • Deoxyribonucleotides
  • Polynucleotides
  • Tritium
  • Manganese
  • RNA
  • DNA
  • DNA Nucleotidyltransferases