Induction of auxotrophic mutations by treatment of Chinese hamster cells with 5-bromodeoxyuridine and black light

Proc Natl Acad Sci U S A. 1972 Nov;69(11):3459-63. doi: 10.1073/pnas.69.11.3459.


Treatment of an aneuploid Chinese hamster cell line (V79) in tissue culture with 5-bromodeoxyuridine and black light, with or without prior exposure of cells to another mutagen, led to the isolation of auxotrophic mutants, each of which exhibits a specific nutritional requirement for glycine, uridine, purine, or for a combination of glycine, hypoxanthine, and thymidine. 61 mutants that could not use exogenous galactose were also isolated. Thus, this treatment functions as an inductive, but not as a selective, procedure for mutations in mammalian cells.

MeSH terms

  • Animals
  • Azaguanine / pharmacology
  • Bromodeoxyuridine / pharmacology*
  • Cell Line
  • Cell Survival
  • Cricetinae
  • Culture Media
  • Fluorescence*
  • Galactose / metabolism
  • Glucose / metabolism
  • Glycine / metabolism
  • Hypoxanthines / metabolism
  • Mutagens*
  • Mutation / drug effects
  • Thymidine / metabolism
  • Uridine / metabolism


  • Culture Media
  • Hypoxanthines
  • Mutagens
  • Bromodeoxyuridine
  • Glucose
  • Azaguanine
  • Glycine
  • Thymidine
  • Uridine
  • Galactose