Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 70 (2), 485-9

Insulin-like Activity of Concanavalin A and Wheat Germ Agglutinin--Direct Interactions With Insulin Receptors

Insulin-like Activity of Concanavalin A and Wheat Germ Agglutinin--Direct Interactions With Insulin Receptors

P Cuatrecasas et al. Proc Natl Acad Sci U S A.

Abstract

Concanavalin A and wheat germ agglutinin are as effective as insulin in enhancing the rate of glucose transport and in inhibiting epinephrine-stimulated lipolysis in isolated adipocytes. These lectins, also like insulin, inhibit basal as well as epinephrine-stimulated adenylate cyclase activity of membranes obtained from homogenates of fat cells. Low concentrations of wheat germ agglutinin enhance the specific binding of insulin to receptors of fat cells and liver membranes. Higher concentrations of this plant lectin, as well as of concanavalin A, competitively displace the binding of insulin to receptors in these tissues. These effects are equally apparent in insulin-binding proteins solubilized from membranes, indicating that the plant lectins interact directly with insulin receptors. All of the effects observed with the plant lectins are reversed by simple sugars that bind specifically to these plant proteins. Agarose derivatives of the plant lectins effectively adsorb solubilized insulin-binding proteins, and these can be eluted with buffers containing specific simple sugars. The possible implications of these findings to certain biological properties (mitogenicity) of these lectins and to the mechanism of action of other growth-promoting substances are considered.

Similar articles

See all similar articles

Cited by 53 PubMed Central articles

See all "Cited by" articles

References

    1. Biochemistry. 1972 Jun 6;11(12):2291-9 - PubMed
    1. J Biol Chem. 1970 Jun;245(12):3059-65 - PubMed
    1. Arch Biochem Biophys. 1968 Sep 20;127(1):406-12 - PubMed
    1. J Biol Chem. 1969 Oct 10;244(19):5181-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1648-52 - PubMed

MeSH terms

Feedback