Erythrosin B inhibits dopamine transport in rat caudate synaptosomes

Science. 1979 Jul 27;205(4404):410-2. doi: 10.1126/science.451609.

Abstract

Erythrosin B is a member of a class of fluorescein dyes that are suggested to elicit hyperkinesis when ingested by susceptible children. We found that erythrosin B inhibits dopamine uptake in rat caudate synaptosomes "uncompetitively" in the 10- to 800-micromolar range. Half maximal inhibition of uptake occurred at 45 micromolar. Uncompetitive inhibition denotes a decrease in efficacy of the dopamine membrane transport mechanism with an increase in affinity of dopamine to the carrier. Erythrosin B also decreased nonsaturable binding of dopamine to the synaptosome membrane. The inhibitory action of erythrosin B on dopamine uptake is consistent with the hypothesis that erythrosin B can act as a central excitatory agent able to induce hyperkinetic behavior.

MeSH terms

  • Animals
  • Biological Transport / drug effects
  • Caudate Nucleus / drug effects
  • Caudate Nucleus / metabolism*
  • Dopamine / metabolism*
  • Erythrosine / pharmacology*
  • Fluoresceins / pharmacology*
  • Kinetics
  • Rats
  • Synaptosomes / drug effects
  • Synaptosomes / metabolism*

Substances

  • Fluoresceins
  • Erythrosine
  • Dopamine