Transfer RNA biosynthesis: the nucleotide sequence of a precursor to serine and proline transfer RNAs

Proc Natl Acad Sci U S A. 1974 Feb;71(2):413-6. doi: 10.1073/pnas.71.2.413.


The nucleotide sequence of a transfer RNA precursor molecule coded by bacteriophage T4 has been determined. The molecule is a single polynucleotide chain which contains two transfer RNA species that are destined to recognize serine and proline. The 3' -CCA(OH) termini of both mature transfer RNA species are absent in the precursor molecule; these termini must therefore be added enzymatically at a subsequent stage of maturation. Nucleotide residues unique to the precursor are located at both ends of the molecule and between the two transfer RNA sequences.

MeSH terms

  • Base Sequence
  • Coliphages / metabolism*
  • Lysogeny
  • Polynucleotides / analysis*
  • Polynucleotides / metabolism
  • Proline
  • RNA, Transfer / biosynthesis*
  • RNA, Viral / biosynthesis*
  • Serine


  • Polynucleotides
  • RNA, Viral
  • Serine
  • RNA, Transfer
  • Proline

Associated data

  • GENBANK/M10874