Adriamycin and daunorubicin inhibition of mutant T4 DNA polymerases

Proc Natl Acad Sci U S A. 1974 Apr;71(4):1193-6. doi: 10.1073/pnas.71.4.1193.


The anticancer drugs, adriamycin and daunorubicin, as well as two other DNA reagents, ethidium bromide and 9-aminoacridine, all exert a differential inhibitory effect on nucleotide incorporation for purified DNA polymerases induced by mutant and wild-type bacteriophage T4. When compared with DNA polymerase of wild-type phage, antimutator enzymes are inhibited to a far greater extent and mutator enzymes to a lesser extent. In contrast, the polymerase-associated 3'-exonuclease activities of wild type and mutants are also inhibited by the compounds but nondifferentially.

MeSH terms

  • Acridines / pharmacology
  • Coliphages / enzymology*
  • DNA Nucleotidyltransferases / antagonists & inhibitors*
  • DNA Nucleotidyltransferases / metabolism
  • DNA Viruses
  • Daunorubicin / pharmacology*
  • Doxorubicin / pharmacology*
  • Enzyme Activation / drug effects
  • Ethidium / pharmacology
  • Exonucleases / antagonists & inhibitors
  • Mutation*
  • Nucleotides / metabolism


  • Acridines
  • Nucleotides
  • Doxorubicin
  • DNA Nucleotidyltransferases
  • Exonucleases
  • Ethidium
  • Daunorubicin