Seven healthy young adults were maintained for two separate 1-week periods on each of two very high-carbohydrate diets, one with low-fiber and one with high-fiber content. In both diets 15% of the calories were from protein, 15% from fat, and 70% were from carbohydrate. The low-fiber diet consisted of milk, glucose, and dextrins in liquid formula form, the high-fiber diet was composed of starchy foods. The crude fiber content of the high- and low-fiber diets was 18.0 and 1.0 g, respectively. The diets were isocaloric and the subjects maintained a stable weight. During the low-fiber diet the fasting triglycerides rose, reaching a peak 45% above base-line in 6 days. During the high-fiber diet the triglycerides fell to a level slightly below base-line. The cholesterol fell 16 and 23% below base-line on the low- and high-fiber diets. The glucose response to test meals representative of each diet was similar. The insulin response to a low-fiber meal was twice as great as that to a high-fiber meal containing an equivalent amount of carbohydrate. The results suggest that carbohydrate-induced hyperlipemia does not occur if the high carbohydrate diet is rich in dietary fiber, and furthermore that the insulin-stimulating potential of foods in a very high-carbohydrate diet is a critical determinant of the magnitude of carbohydrate-induced lipemia.