Formation of catenated colicin factor E1 deoxyribonucleic acid molecules in a recombination-deficient strain of Escherichia coli

J Bacteriol. 1973 Feb;113(2):1084-5. doi: 10.1128/jb.113.2.1084-1085.1973.

Abstract

Catenated deoxyribonucleic acid molecules of colicin factor E1 (Col E1) were found with nearly equal frequency in minicells derived from Escherichia coli strain P678-54 recA(+) (Col E1) and P678-54 recA(-) (Col E1). The result suggests that the recombination function controlled by the recA gene does not influence the formation of catenated Col E1.

MeSH terms

  • Centrifugation, Density Gradient
  • Colicins / biosynthesis*
  • Conjugation, Genetic
  • DNA Replication
  • DNA, Bacterial / analysis
  • DNA, Bacterial / biosynthesis*
  • DNA, Bacterial / isolation & purification
  • DNA, Circular / analysis
  • DNA, Circular / isolation & purification
  • Escherichia coli / cytology
  • Escherichia coli / metabolism*
  • Genes
  • Microscopy, Electron
  • Nucleic Acid Conformation
  • Recombination, Genetic*
  • Thymidine / metabolism
  • Tritium

Substances

  • Colicins
  • DNA, Bacterial
  • DNA, Circular
  • Tritium
  • Thymidine