Abstract
Catenated deoxyribonucleic acid molecules of colicin factor E1 (Col E1) were found with nearly equal frequency in minicells derived from Escherichia coli strain P678-54 recA(+) (Col E1) and P678-54 recA(-) (Col E1). The result suggests that the recombination function controlled by the recA gene does not influence the formation of catenated Col E1.
MeSH terms
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Centrifugation, Density Gradient
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Colicins / biosynthesis*
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Conjugation, Genetic
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DNA Replication
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DNA, Bacterial / analysis
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DNA, Bacterial / biosynthesis*
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DNA, Bacterial / isolation & purification
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DNA, Circular / analysis
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DNA, Circular / isolation & purification
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Escherichia coli / cytology
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Escherichia coli / metabolism*
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Genes
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Microscopy, Electron
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Nucleic Acid Conformation
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Recombination, Genetic*
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Thymidine / metabolism
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Tritium
Substances
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Colicins
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DNA, Bacterial
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DNA, Circular
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Tritium
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Thymidine