Induction of B cell tolerance in vitro to 2,4-dinitrophenyl coupled to a copolymer of D-glutamic acid and D-lysine (DNP-D-GL)

J Exp Med. 1973 Jul 1;138(1):312-7. doi: 10.1084/jem.138.1.312.

Abstract

Spleen cells from CBA or congenitally athymic ("nude") mice were pretreated with various concentrations of DNP coupled to a copolymer of D-glutamic acid and D-lysine (DNP(37)-D-GL), under various conditions of time and temperature. After washing, they were then cultured for 3 days with the direct B cell immunogen, DNP coupled to Salmonella adelaide flagella (DNP-FLA). Under all circumstances tried, exposure of cells to 1 microg/ml DNP-D-GL caused a 70-100% depression in the subsequent DNP-specific PFC response, and 100 ng/ml caused a lesser but still substantial effect. At the concentrations used, DNP-D-GL did not affect irrelevant antibody responses. Though cells from nude mice responded somewhat less well to DNP-FLA than those from CBA mice, no significant difference in the reaction of the two populations to the tolerogen was noted. This demonstrates that DNP-D-GL can, as previously suspected, directly cause unresponsiveness in B lymphocytes.

MeSH terms

  • Animals
  • Antibody-Producing Cells
  • Antigens, Bacterial
  • B-Lymphocytes / immunology*
  • Binding Sites, Antibody
  • Cells, Cultured
  • Dinitrophenols*
  • Flagella / immunology
  • Glutamates*
  • Haptens
  • Hemolytic Plaque Technique
  • Immune Tolerance*
  • Lysine*
  • Male
  • Mice
  • Salmonella / immunology
  • Spleen / immunology

Substances

  • Antigens, Bacterial
  • Dinitrophenols
  • Glutamates
  • Haptens
  • Lysine