Fluorescent labelling of proteins of lymphocyte plasma membranes

J Immunol Methods. 1979 May 10;27(1):55-9. doi: 10.1016/0022-1759(79)90238-2.

Abstract

Fluorescamine has been used for labelling proteins present on the surface of normal human peripheral blood lymphocytes. Under the conditions of study, 12 labelled proteins could be detected by SDS gel electrophoresis. This method may be of value in biochemical studies of lymphocyte membranes.

MeSH terms

  • Antigens, Surface* / isolation & purification
  • Cell Membrane / immunology
  • Fluorescamine / pharmacology
  • Humans
  • Lymphocytes / cytology*
  • Lymphocytes / immunology
  • Lymphocytes / metabolism
  • Proteins / metabolism
  • Spectrometry, Fluorescence

Substances

  • Antigens, Surface
  • Proteins
  • Fluorescamine