In vitro repair of apurinic sites in DNA

Proc Natl Acad Sci U S A. 1974 Jun;71(6):2273-5. doi: 10.1073/pnas.71.6.2273.

Abstract

Apurinic sites disappear from DNA during an incubation of the DNA with the Escherichia coli endonuclease specific for apurinic sites, DNA polymerase I (EC 2.7.7.7.), and T4 ligase (EC 6.5.1.1). Omission of any one of these three enzymes and, in particular, omission of the endonuclease specific for apurinic sites prevents this in vitro repair.

MeSH terms

  • Binding Sites
  • Coliphages / metabolism*
  • DNA Nucleotidyltransferases / metabolism
  • DNA Repair*
  • DNA Viruses / metabolism
  • DNA, Viral / metabolism
  • Endonucleases / metabolism
  • Escherichia coli
  • Nucleic Acid Denaturation
  • Polynucleotide Ligases / metabolism

Substances

  • DNA, Viral
  • DNA Nucleotidyltransferases
  • Endonucleases
  • Polynucleotide Ligases