Abstract
A specific lipoprotein of the E. coli outer membrane has been synthesized in a cell-free system directed by purified messenger RNA. The mRNA for the lipoprotein was purified as 7S RNA about 250-fold from exponentially growing cells. Protein synthesis of the cell-free system was totally dependent upon the addition of the purified mRNA. The product of the cell-free system was identified as the specific lipoprotein by immunoprecipitation and by peptide mapping.
MeSH terms
-
Amino Acid Sequence
-
Bacterial Proteins / biosynthesis*
-
Cell Membrane / metabolism
-
Cell-Free System
-
Chromatography, Gel
-
Cyanogen Bromide
-
Electrophoresis, Polyacrylamide Gel
-
Escherichia coli / ultrastructure*
-
Hydrolysis
-
Lipoproteins / biosynthesis*
-
Lipoproteins / immunology
-
Methionine / metabolism
-
Molecular Weight
-
Peptide Fragments / analysis
-
Precipitin Tests
-
RNA, Bacterial / isolation & purification
-
RNA, Bacterial / metabolism*
-
RNA, Messenger / isolation & purification
-
RNA, Messenger / metabolism*
-
Sulfur Radioisotopes
Substances
-
Bacterial Proteins
-
Lipoproteins
-
Peptide Fragments
-
RNA, Bacterial
-
RNA, Messenger
-
Sulfur Radioisotopes
-
Methionine
-
Cyanogen Bromide