Liver specific antigens. Purification and characterization

Clin Exp Immunol. 1972 Jan;10(1):89-102.

Abstract

Human liver has been homogenated and submitted to 150,000 g in order to investigate the supernatant for the presence of liver specific antigens. Gel-filtration on Sephadex G-100 revealed liver specific antigenicity in the first peak. Gel-filtration of the first peak on Sephadex G-200 revealed two peaks each exhibiting liver specific antigenicity. Immunochemical and physicochemical analysis made it possible to define the liver-specific antigen present in the first peak as a lipoprotein with the characteristics of a high molecular weight, low density protein. It is partly located in the membrane of liver cells. The liver specific protein of the second Sephadex G-200 peak has a molecular weight of about 190,000. It is located within the cytoplasm of hepatocytes.

MeSH terms

  • Acrylates
  • Animals
  • Antigens / isolation & purification*
  • Centrifugation, Density Gradient
  • Chromatography, Gel
  • Complement Fixation Tests
  • Cytoplasm / analysis
  • Dextrans
  • Electrophoresis
  • Fluorescent Antibody Technique
  • Humans
  • Immune Sera
  • Immunoassay
  • Immunodiffusion
  • Immunoelectrophoresis
  • Lipoproteins / isolation & purification
  • Liver / immunology*
  • Molecular Weight
  • Proteins / isolation & purification
  • Rabbits

Substances

  • Acrylates
  • Antigens
  • Dextrans
  • Immune Sera
  • Lipoproteins
  • Proteins