Simultaneous demonstration of lipids and starch in plant tissues

Stain Technol. 1975 Jan;50(1):1-4. doi: 10.3109/10520297509117023.

Abstract

A rapid and easy technique for the simultaneous demonstration of lipids and starch in the same histological section is described. Tissues are prepared by the classical fixing and Araldite M embedding techniques of electron microscopy. Semithin sections are directly stained for 1 hour at 60C with saturated Sudan black B in 70% ethanol without removing the embedding resin. Lipids stain black; starch is shown as white grains contrasting with the blue-grey embedding resin.

MeSH terms

  • Histological Techniques
  • Lipids / analysis*
  • Microscopy, Electron
  • Plants / analysis*
  • Resins, Plant
  • Staining and Labeling / methods
  • Starch / analysis*

Substances

  • Lipids
  • Resins, Plant
  • Starch