Alkyl isocyanates as active-site-specific reagents for serine proteases. Identification of the active-site serine as the site of reaction

MeSH terms

• Amino Acids / analysis
• Binding Sites
• Carbamates
• Carbon Isotopes
• Chromatography, Ion Exchange
• Chymotrypsin* / analysis
• Chymotrypsin* / antagonists & inhibitors
• Cyanates*
• Endopeptidases
• Formates
• Hydrolysis
• Oxidation-Reduction
• Pancreatic Elastase* / analysis
• Pancreatic Elastase* / antagonists & inhibitors
• Peptides / analysis
• Peptides / isolation & purification
• Serine* / analysis
• Subtilisins
• Trypsin