Morphological, biochemical, and growth characteristics of pseudomonas cepacia from distilled water

Appl Microbiol. 1973 Mar;25(3):476-83.

Abstract

Studies were conducted on three strains of Pseudomonas cepacia isolated and maintained in distilled water and on a laboratory-subcultured strain transferred to distilled water. Optimum growth rates and maximum population yields of the four strains in distilled water were obtained at 37 C, although high population levels (10(6)-10(7)/ml) were reached and maintained over extended incubation periods at temperatures from 18 C to 42 C. Two strains were able to grow in distilled water at temperatures ranging from 12 C to 48 C and to survive 48 h and 21 days at 50 C and 10 C, respectively. Cells from distilled water cultures inoculated into Trypticase soy broth showed an immediate two- to three-log drop at upper and lower temperature limits; survivors were able to initiate logarithmic growth. Results obtained in morphological, biochemical, and antibiotic tests affirmed the strain differences noted in growth studies.

MeSH terms

  • Aerobiosis
  • Anti-Bacterial Agents / pharmacology
  • Carbohydrate Metabolism
  • Carboxy-Lyases / biosynthesis
  • Cell Survival
  • Culture Media
  • Drug Resistance, Microbial
  • Flagella
  • Gelatin / metabolism
  • Hospitals
  • Lysine
  • Microbial Sensitivity Tests
  • Microscopy, Electron
  • Nitrates / metabolism
  • Pseudomonas* / cytology
  • Pseudomonas* / drug effects
  • Pseudomonas* / enzymology
  • Pseudomonas* / growth & development
  • Pseudomonas* / isolation & purification
  • Pseudomonas* / metabolism
  • Temperature
  • Time Factors
  • Urease / biosynthesis
  • Water Microbiology*

Substances

  • Anti-Bacterial Agents
  • Culture Media
  • Nitrates
  • Gelatin
  • Urease
  • Carboxy-Lyases
  • Lysine