Isolation of adenovirus type 5 host range deletion mutants defective for transformation of rat embryo cells

Cell. 1979 Jul;17(3):683-9. doi: 10.1016/0092-8674(79)90275-7.

Abstract

A series of adenovirus type 5 (Ad5) deletion, insertion and substitution mutants, some of which are defective for transformation of rat cells, have been isolated. The mutants were selected as variants which lack the Xba I endonuclease cleavage site at 4 map units on the viral chromosome. The deletions range in size from 150-2300 bp and are located between 1.5 and 10.5 map units. The mutants can be propagated in 293 cells (Ad5-transformed human embryonic kidney cells), but are defective for growth in HeLa or human embryonic kidney cells. No viral DNA synthesis was observed in mutant virus-infected HeLa cells. All but one of the deletion mutants tested were defective for transformation of rat embryo and rat embryo brain cells.

MeSH terms

  • Adenoviridae / genetics*
  • Animals
  • Cell Transformation, Neoplastic*
  • Cells, Cultured
  • DNA Restriction Enzymes / pharmacology
  • DNA, Viral / biosynthesis
  • Embryo, Mammalian
  • Genes, Viral*
  • Genetic Complementation Test
  • HeLa Cells
  • Humans
  • Kidney
  • Mutation*
  • Phenotype
  • Rats

Substances

  • DNA, Viral
  • DNA Restriction Enzymes