Extraction of the reduced nicotinamide adenine dinucleotide phosphate:delta 4-3-ketosteroid-5-alpha oxidoreductase of rat prostate with digitonin and potassium chloride

Biochemistry. 1974 Jan 29;13(3):450-6. doi: 10.1021/bi00700a009.
No abstract available

MeSH terms

  • Animals
  • Castration
  • Cell Nucleus / drug effects
  • Cell Nucleus / enzymology*
  • Centrifugation, Density Gradient
  • Digitonin / pharmacology
  • Drug Stability
  • Glycerol
  • Hydrogen-Ion Concentration
  • Male
  • Membranes / enzymology
  • Microsomes / drug effects
  • Microsomes / enzymology*
  • Molecular Weight
  • Oxidoreductases / isolation & purification*
  • Potassium Chloride / pharmacology
  • Propionates / pharmacology
  • Prostate / cytology
  • Prostate / drug effects
  • Prostate / enzymology*
  • Rats
  • Solubility
  • Testosterone / pharmacology

Substances

  • Propionates
  • Testosterone
  • Potassium Chloride
  • Oxidoreductases
  • Digitonin
  • Glycerol